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FIG. 1. Sequence alignment of 12 strains of B. adolescentis with the database sequence, showing sequence differences. Satokari et al. re- ported that B. adolescentis E-981074T (ATCC <t>15703T)</t> harbors five copies of the rrn operon, and the sequences AF275881 (nru-1) and AF275882 (nru-5) differed at several base pairs (28). The annealing sites for the B. adolescentis-specific forward primer are boxed. Nucle- otides differing from the type strain of B. adolescentis are shaded. These strains were divided into two genotypes, with six strains of genotype A (ATCC 15703T, MC-39, A-001, B-002, C-001, and <t>D-027a)</t> and seven strains of genotype B (MC-36, MC-37, MC-38, MC-41, D-009, E-040, and E-067).
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FIG. 1. Sequence alignment of 12 strains of B. adolescentis with the database sequence, showing sequence differences. Satokari et al. re- ported that B. adolescentis E-981074T (ATCC <t>15703T)</t> harbors five copies of the rrn operon, and the sequences AF275881 (nru-1) and AF275882 (nru-5) differed at several base pairs (28). The annealing sites for the B. adolescentis-specific forward primer are boxed. Nucle- otides differing from the type strain of B. adolescentis are shaded. These strains were divided into two genotypes, with six strains of genotype A (ATCC 15703T, MC-39, A-001, B-002, C-001, and <t>D-027a)</t> and seven strains of genotype B (MC-36, MC-37, MC-38, MC-41, D-009, E-040, and E-067).
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FIG. 1. Sequence alignment of 12 strains of B. adolescentis with the database sequence, showing sequence differences. Satokari et al. re- ported that B. adolescentis E-981074T (ATCC <t>15703T)</t> harbors five copies of the rrn operon, and the sequences AF275881 (nru-1) and AF275882 (nru-5) differed at several base pairs (28). The annealing sites for the B. adolescentis-specific forward primer are boxed. Nucle- otides differing from the type strain of B. adolescentis are shaded. These strains were divided into two genotypes, with six strains of genotype A (ATCC 15703T, MC-39, A-001, B-002, C-001, and <t>D-027a)</t> and seven strains of genotype B (MC-36, MC-37, MC-38, MC-41, D-009, E-040, and E-067).
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FIG. 1. Sequence alignment of 12 strains of B. adolescentis with the database sequence, showing sequence differences. Satokari et al. re- ported that B. adolescentis E-981074T (ATCC <t>15703T)</t> harbors five copies of the rrn operon, and the sequences AF275881 (nru-1) and AF275882 (nru-5) differed at several base pairs (28). The annealing sites for the B. adolescentis-specific forward primer are boxed. Nucle- otides differing from the type strain of B. adolescentis are shaded. These strains were divided into two genotypes, with six strains of genotype A (ATCC 15703T, MC-39, A-001, B-002, C-001, and <t>D-027a)</t> and seven strains of genotype B (MC-36, MC-37, MC-38, MC-41, D-009, E-040, and E-067).
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FIG. 1. Sequence alignment of 12 strains of B. adolescentis with the database sequence, showing sequence differences. Satokari et al. re- ported that B. adolescentis E-981074T (ATCC <t>15703T)</t> harbors five copies of the rrn operon, and the sequences AF275881 (nru-1) and AF275882 (nru-5) differed at several base pairs (28). The annealing sites for the B. adolescentis-specific forward primer are boxed. Nucle- otides differing from the type strain of B. adolescentis are shaded. These strains were divided into two genotypes, with six strains of genotype A (ATCC 15703T, MC-39, A-001, B-002, C-001, and <t>D-027a)</t> and seven strains of genotype B (MC-36, MC-37, MC-38, MC-41, D-009, E-040, and E-067).
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FIG. 1. Sequence alignment of 12 strains of B. adolescentis with the database sequence, showing sequence differences. Satokari et al. re- ported that B. adolescentis E-981074T (ATCC <t>15703T)</t> harbors five copies of the rrn operon, and the sequences AF275881 (nru-1) and AF275882 (nru-5) differed at several base pairs (28). The annealing sites for the B. adolescentis-specific forward primer are boxed. Nucle- otides differing from the type strain of B. adolescentis are shaded. These strains were divided into two genotypes, with six strains of genotype A (ATCC 15703T, MC-39, A-001, B-002, C-001, and <t>D-027a)</t> and seven strains of genotype B (MC-36, MC-37, MC-38, MC-41, D-009, E-040, and E-067).
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FIG. 1. Sequence alignment of 12 strains of B. adolescentis with the database sequence, showing sequence differences. Satokari et al. re- ported that B. adolescentis E-981074T (ATCC 15703T) harbors five copies of the rrn operon, and the sequences AF275881 (nru-1) and AF275882 (nru-5) differed at several base pairs (28). The annealing sites for the B. adolescentis-specific forward primer are boxed. Nucle- otides differing from the type strain of B. adolescentis are shaded. These strains were divided into two genotypes, with six strains of genotype A (ATCC 15703T, MC-39, A-001, B-002, C-001, and D-027a) and seven strains of genotype B (MC-36, MC-37, MC-38, MC-41, D-009, E-040, and E-067).

Journal: Applied and Environmental Microbiology

Article Title: Quantitative PCR with 16S rRNA-Gene-Targeted Species-Specific Primers for Analysis of Human Intestinal Bifidobacteria

doi: 10.1128/aem.70.1.167-173.2004

Figure Lengend Snippet: FIG. 1. Sequence alignment of 12 strains of B. adolescentis with the database sequence, showing sequence differences. Satokari et al. re- ported that B. adolescentis E-981074T (ATCC 15703T) harbors five copies of the rrn operon, and the sequences AF275881 (nru-1) and AF275882 (nru-5) differed at several base pairs (28). The annealing sites for the B. adolescentis-specific forward primer are boxed. Nucle- otides differing from the type strain of B. adolescentis are shaded. These strains were divided into two genotypes, with six strains of genotype A (ATCC 15703T, MC-39, A-001, B-002, C-001, and D-027a) and seven strains of genotype B (MC-36, MC-37, MC-38, MC-41, D-009, E-040, and E-067).

Article Snippet: As shown in Fig. 1, these strains were divided into two genotypes, with six strains of genotype A (ATCC 15703T, MC-39, A-001, B-002, C-001, and D-027a) and seven strains of genotype B (MC-36, MC-37, MC-38, MC-41, D-009, E-040, and E-067).

Techniques: Sequencing